利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN104745684A 一种检测污水中亚硝酸盐氧化菌群落结构和丰度的方法 无效 - 驳回

一种检测污水中亚硝酸盐氧化菌群落结构和丰度的方法
摘要：
本发明公开一种测污水中亚硝酸盐氧化菌群落结构和丰度的方法，具体步骤为：（a）提取污水中活性污泥的全部基因组DNA；（b）分别对nxrA基因和nxrB基因进行PCR扩增；（c）以罗氏454焦磷酸测序法分别对扩增产物进行测序；（d）对测序结果划分操作分类单元；（e）选取具有97%相似度的操作分类单元序列进行BLASTn比对，利用MEGA软件分别对nxrA基因和nxrB基因划分系统进化树，即为污水中亚硝酸盐氧化菌的群落结构；nxrA基因和nxrB基因的不同操作分类单元的相对丰度即为污水中亚硝酸盐氧化菌的丰度；该方法操作简便，检测范围广，既可应用于实验室的检测分析，也可以实现对污水厂脱氮效率的实时监测。
摘要（英）：

The invention discloses a method for detecting the community structures and abundance of nitrite oxidizing bacteria in wastewater. The method comprises the concrete steps as follows: (a) extracting the whole-genome DNA of activated sludge in the wastewater; (b) performing PCR amplification of a gene nxrA and a gene nxrB; (c) sequencing amplification products respectively by a Roche 454 pyrosequencing method; (d) dividing the OTUs (Operational Taxonomic Units) of sequencing results; and (e) selecting OTUs sequences with the similarity of 97%, performing BLASTn comparison between the OTUs sequences, and dividing the phylogenetic trees of the gene nxrA and the gene nxrB respectively by using MEGA software, wherein the phylogenetic trees are the community structures of the nitrite oxidizing bacteria in the wastewater, and the relative abundance of the different OTUs of the gene nxrA and the gene nxrB is the abundance of the nitrite oxidizing bacteria in the wastewater. The method is simple and convenient to operate, has a wide detection range, can be applied to detection and analysis in a laboratory and can also realize real-time detection of the nitrogen removal efficiency of a wastewater treatment plant.

0/0