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发明公开
CN104755625A 使用突变的胱硫醇γ-合酶从O-磷酸-L-高丝氨酸和甲硫醇制备L-甲硫氨酸的方法 有权

使用突变的胱硫醇γ-合酶从O-磷酸-L-高丝氨酸和甲硫醇制备L-甲硫氨酸的方法
摘要：
本发明提供了用于制备L-甲硫氨酸的方法，其中O-磷酸-L-高丝氨酸和甲硫醇通过酶转化为L-甲硫氨酸和H3PO4。所述转化通过称为依赖O-磷酸-L-高丝氨酸(OHPS)的甲硫氨酸合酶实现。还描述了依赖O-磷酸-L-高丝氨酸(OHPS)的甲硫氨酸合酶，即能将O-磷酸-L-高丝氨酸和甲硫醇通过酶转化为L-甲硫氨酸和H3PO4的蛋白质以及已经过基因修饰从而能从O-磷酸-L-高丝氨酸和甲硫醇制备L-高丝氨酸的微生物。还描述了用于筛选催化O-磷酸-L-高丝氨酸和甲硫醇至L-甲硫氨酸和H3PO4的转化的酶的方法。
摘要（英）：

Provided is a method for producing L-methionine in which O-phospho-L-homoserine and methanethiol are enzymatically converted into L-methionine and H3P04. Such a conversion is achieved by an enzyme called O-phospho-L-homoserine (OHPS) dependent methionine synthase. Also described are O-phospho-L-homoserine (OHPS) dependent methionine synthases, i.e. proteins which are able to enzymatically convert O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4 as well as microorganisms which have been genetically modified so as to be able to produce L-methionine from O-phospho-L-homoserine and methanethiol. Furthermore described are methods to screen for enzymes that catalyse the conversion of O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4.

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