The invention provides a competitive ELISA detection method for DHAV-1 antibodies. The detection method solves the problem that at present, the valence of the DHAV-1 antibodies in the bodies of breed ducks and ducklings and the valence of commercial DHAV-1 egg yolk antibodies are difficult to evaluate. The detection method includes the steps of performing monoclonal antibody making, antigen coating, washing, closing, washing, enzyme-labeled antibody treating, washing and TMB developing; determining the OD450 nm value of each reaction hole; calculating the inhibition ratio of a serum sample, wherein the inhibition ratio (%)=(the OD value of an enzyme-labeled antibody-the OD value of the sample)*100%/the OD value of the enzyme-labeled antibody; if PI>=15.21%, judging that serum is positive; if PI<15.21%, judging that the serum is negative. According to the competitive ELISA detection method for the DHAV-1 antibodies, high specificity of monoclonal antibodies and high sensitivity of ELISA tests are achieved, stability of results is good, commercialization and automation can be achieved, and judgment can be made by naked-eye observation.