The invention discloses a blood taking needle for an experimental animal heart. The blood taking needle comprises a needle head, wherein the needle head mainly comprises a needle stem in the shape of a slender tube, a needle tip at the front end of the needle stem and a needle bolt at the rear end of the needle stem; the needle tip is in the shape of a flat circle; a limiting device for limiting the needle head feeding depth is arranged on the tube wall of the needle stem. According to the blood taking needle for the experimental animal heart disclosed by the invention, the accuracy of a blood taking point can be guaranteed by taking samples from different experimental animal heats, injuries caused by the blood taking needle are effectively reduced, and a fatality rate is reduced to a great extent.