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发明公开
CN104774915A 一种催化灯标及其制备方法以及用催化灯标测定痕量铀的方法 无效 - 撤回

一种催化灯标及其制备方法以及用催化灯标测定痕量铀的方法
摘要：
本发明公开了一种催化灯标及其制备方法以及用催化灯标测定痕量铀的方法。催化灯标由DNA酶和底物DNA两部分组成，DNA酶序列的3’末端为连续的4个鸟嘌呤脱氧核苷酸（G），底物DNA的序列中间具有一个腺嘌呤核苷酸（rA），底物DNA的5’末端用4-甲基-6-羧基罗丹明（TAMRA）荧光染料修饰。将DNA酶与底物DNA混合杂交形成所述催化灯标，加入到待测样品体系中反应，测定已知浓度硝酸铀酰离子的荧光强度和样品的荧光强度并计算可得到样品中的铀含量。本发明是一种快速、准确、高灵敏度和高选择性测定痕量铀的方法。
摘要（英）：

The invention discloses a catalytic light mark and a preparation method thereof, and a method for determination of trace uranium by the catalytic light mark. The catalytic light mark is composed of two parts of a DNA enzyme and a substrate DNA, a 3' end of a DNA enzyme sequence comprises four continuous guanine deoxynucleotides (G), the middle of a substrate DNA sequence has an adenine nucleotide (rA), and a 5' end of the substrate DNA is modified by a 4-methyl-6-carboxyl rhodamine (TAMRA) fluorescent dye. The DNA enzyme and the substrate DNA are in mixing hybridization to form the catalytic light mark, the catalytic light mark is added to a reaction system of a to-be-measured sample to undergo a reaction, the fluorescence intensity of uranyl nitrate ions with known concentration and the fluorescence intensity of the sample are determined, and the uranium content in the sample can be obtained through calculation. The method is a fast, accurate, high-sensitivity and high-selectivity method for determination of trace uranium.

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