The invention discloses a water-containing thickened oil PVT experiment method. The method comprises the following steps of carrying out physical property analysis on water-containing emulsified oil, respectively metering a volume and quality of water-containing stratum thickened oil by conventional methods, releasing all the water-containing thickened oil into an atmospheric environment, carrying out water content determination on the crude oil, carrying out oil-water separation, determining oil and water density and calculating real physical property parameters of the stratum crude oil according to a derived formula. The method realizes analysis of thickened oil with water content more than 5% and realizes accurate sampling and analysis on high-water content thickened oil well. The method is suitable for water-containing thickened oil and ultra-thick oil.