The invention discloses a novel information security attack and defense experiment platform and an implementation method thereof. The attack and defense experiment platform is divided into four layers: a resource layer, a data layer, an interface layer and a business layer, and corresponding subsystems are formed; through the linkage of all the subsystems in the attack and defense experiment platform, efficient intelligence collection can be performed, a virtual attack and defense exercise scene is rapidly formed, and information security antagonism is simulated on the scene, and the like. According to the attack and defense experiment platform disclosed by the invention, the automation configuration technology, the virtualization technology and a resource abstract management method are adopted in the resource layer, so a uniform source management method for the combination of virtual hardware and entity hardware is realized, and the practical problem that the rapid change requirement of the information security field cannot be met due to the fact that hardware resources are high in input, hard to manage, low in efficiency, high in energy consumption, low in flexibility, repeated in investment, difficult in configuration, and the like in the traditional experiment environment is solved.