The invention discloses matrix for tobacco axillary bud cutting propagation and application thereof. The matrix is composed of a haycite part A and a mixed matrix body part B. The mixed matrix body is composed of, by volume, 50%-60% of grass carbon, 20%-30% of perlite, 10%-20% of carbonized chaff and 5%-10% of humic acid. According to the matrix for tobacco axillary bud cutting propagation and application thereof, cutting propagation of tobacco axillary buds is conducted through the matrix, according to the matrix environmental requirement for root development of the cut tobacco axillary buds, the matrix is divided into the haycite part and the mixed matrix body part composed of the grass carbon, the perlite, the carbonized chaff and the humic acid after experiment and researches are conducted by inventers, when the matrix is used, the haycite is arranged on the mixed matrix body to play supporting, fixing, dewatering and ventilating functions on the tobacco axillary buds, the mixed matrix body supplies nutrient substance to growth of the tobacco axillary buds, root germination and growth are promoted, and the matrix is divided into parts, so that a higher success rate is achieved for tobacco axillary bud cutting, and meanwhile the growth period of tobacco seedlings is remarkably shortened compared with seed seedling culturing.