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发明公开
CN104789657A TaqMan实时荧光定量PCR检测伊丽莎白巴尔通体的方法 无效 - 驳回

TaqMan实时荧光定量PCR检测伊丽莎白巴尔通体的方法
摘要：
本发明提供了一种TaqMan实时荧光定量PCR检测伊丽莎白巴尔通体的方法，通过选取Be特异的一段基因序列，并基于该特异基因序列设计得到特异性的引物(SEQ ID No.2和SEQ ID No.3)与TaqMan探针(SEQ ID No.4)。本发明建立的TaqMan实时荧光探针定量PCR方法能够在种水平特异性、高灵敏度检出伊丽莎白巴尔通体，为这种巴尔通体所引起的一系列疾病的早期快速诊断、监测和流行病学调查等研究提供有效手段。
摘要（英）：

The invention discloses a method for detecting bartonella elizabethae with TaqMan real-time fluorescent quantitative PCR. The method selects a Be specific gene sequence and obtains specific primers (SEQ ID No.2 and SEQ ID No.3) and a TaqMan probe (SEQ ID No.4) based on the specific gene sequence design. The method can detect the bartonella elizabethae under species level specificity and high sensitivity and provides effective means for researches such as early and rapid diagnosis and monitoring of a series of diseases caused by the bartonella and epidemiological investigation.

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