The invention discloses an improvement method for pre-treatment of Abelson leukemia virus (ALV) test samples. The method comprises: 1, putting a cloacal swab sample, an egg white sample or a cell virus isolated culture sample into a container and marking the samples, and arranging the container provided with the test samples on a finger-shaped tube support; 2, putting the finger-shaped tube support provided with the container into a sealed box, and quickly injecting liquid nitrogen into the sealed box by using a full liquid nitrogen tank, wherein the injected liquid nitrogen should fill the finger-shaped tube support; 3, after nitrogen injection, quickly covering the sealed box with a cover and putting a heavy object on the cover for sealing the sealed box, allowing the sealed box to stand for 1-3 minutes, and opening the cover to check the test samples completely frozen by liquid nitrogen; and 4, taking the frozen test samples out of the box, putting the frozen test samples into a water bath kettle at 36-38 DEG C; warming the samples for 3-5 minutes, and finally performing centrifugation to the warm test sample through a centrifuge at a centrifugal speed of 1000r/min for 30s to obtain the test samples for test.