A regulating control system for a smoking environmental simulation chamber comprises a main body test chamber, an air conditioning mechanism, a humidifying mechanism, and an air change purification mechanism, and is characterized by further comprising a secondary regulating chamber, wherein both the air conditioning mechanism and the humidifying mechanism are communicated with the main body test chamber and the secondary regulating chamber, and can be used for regulating the humiture of the main body test chamber and the secondary regulating chamber simultaneously; the main body test chamber and the secondary regulating chamber are communicated with each other through a pressure regulating mechanism, so that the pressure of the main test chamber is adjustable; an alarming mechanism and a detection control system which is used for testing whether the temperature, humidity and pressure reach the standard or not are arranged in the main body test chamber. Through the adoption of the regulating control system, the humiture and pressure linkage regulating simulation in the smoking environment can be realized, so as to simulate the climate and altitude conditions in different regions, and to overcome the defects that smoking results (cigarette combustion test results, smoking gas index test results and smoking sensory evaluation results) for same cigarettes in different regions are inconsistent due to difference of temperature, humidity, and pressure in smoking environments.