The invention discloses a preparation method of momordica grosvenori and gynostemma pentaphylla tea. The preparation method comprises the following steps of weighing raw medicines; washing; removing impurities; drying and shredding; soaking momordica grosvenori in water; filtering; adding 50% to 90% enthanol into the medicine dregs for reflux extraction; enabling an extraction solution to pass through macro-reticular resin; firstly washing by using 10% to 30% ethanol and then eluting by using 50% to 90% ethanol; drying the elution solution for later use; heating gynostemma pentaphylla in water; heating and extracting by microwave; regulating the pH value to 8.5 to 10.5; filtering; drying filtrate and crushing; uniformly mixing the power to obtain pocket tea, tea soup particles or a tea beverage. The tea is easy to absorb and has the healthcare effects of nourishing and protecting liver, reducing blood fat and the like.