The invention relates to an application of liquids/gas at least containing hydrogen molecules in preparation of a drug resisting to glioma. The glioma resistance effect of the drug is improved due to the application. The drug resisting to glioma prepared through the application comprises the liquids or gas at least containing the hydrogen molecules. The medium can contain gas molecules such as oxygen molecules, carbon dioxide molecules and the like and can also contain liquids such as pure water, deionized water, distilled water, normal saline, acid aqueous solutions of organic acid and the like. The prepared drug resisting to glioma has an inhibition function on growth, infiltration and migration of glioma cells.