The invention discloses a packaging device and a display substrate packaging method and belongs to the technical field of displays. The packaging device comprises a bearing table for bearing a substrate to be packaged, and the bearing table is composed of at least one moving unit capable of moving in a direction perpendicular to the substrate to be packaged. The packaging device can solve the problem that the surface of an OLED (organic light emitting diode) packaged through sheet glue with different surface regions with different deformations forms stripes to affect the display effects, thereby reducing stripes on the surface of the substrate to be packaged and enhancing the display effects. The packaging device and the display substrate packaging method thereof are applied to packaging of display substrates.