A mobile routing inspection device routing inspection line real-time acquisition and adjustment system. An inertial measurement unit sends measured angular speed signal and speed signal of an electric lifting rotating rod to a low-pass filter; the low pass filter performs low-pass filtering on the angular speed signal and the speed signal, and filtered signals are obtained and are sent to a signal processing unit; the signal processing unit extracts angular speed and speed parameters according to the filtered signals, and sends the angular speed and speed parameters to a server side; the server side sends the angular speed and speed parameters to a transformer station monitoring terminal, receives a parameter adjustment command returned by the transformer station monitoring terminal according to the angular speed and speed parameters, and sends the parameter adjustment command to a drive unit; and the drive unit controls motion of the electric lifting rotating rod according to the parameter adjustment command. The mobile routing inspection device routing inspection line real-time acquisition and adjustment system provided by invention does not need rail laying, and can conveniently and easily perform real-time acquisition, setting and adjustment of parameters of a routing inspection line.