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CN104830792A 基于BCG1抗原的DC细胞、靶向性免疫细胞群及其制备方法和用途 无效 - 驳回 转让

基于BCG1抗原的DC细胞、靶向性免疫细胞群及其制备方法和用途
摘要：
本发明公开了DC细胞、靶向性免疫细胞群及其制备方法和用途，其中制备DC细胞的方法包括：将单核细胞进行第一诱导分化培养，以便获得未成熟的DC细胞；利用BCG1抗原mRNA转染所述未成熟的DC细胞，以便获得经过转染的未成熟DC细胞；以及将所述经过转染的未成熟DC细胞进行第二诱导分化培养，以便获得成熟的DC细胞，其中，所述单核细胞是从样本的外周血单个核细胞中分离获得的。利用该发明能够安全高效地制备DC细胞，且本发明的方法成本低、效率高、且能够有效地解决细胞毒性问题，能够有效用于免疫细胞治疗或用于刺激同样患者来源的淋巴细胞产生富含大量CTL细胞的靶向性免疫细胞群，进而用于肿瘤免疫治疗后效果更好。
摘要（英）：

The invention discloses a DC cell based on BCG1 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by BCG1 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

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