利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN104830807A 一株可用于制造疫苗的高效价狂犬疫苗毒株CTN-1V-T 无效 - 撤回

一株可用于制造疫苗的高效价狂犬疫苗毒株CTN-1V-T
摘要：
本发明提供一株具有高效价的狂犬病毒CTN-1V-T vero等细胞适应株；本发明还提供所述狂犬病毒CTN-1V-T在制备vero等细胞纯化灭活狂犬疫苗中的用途。CTN-1V-T是在CTN-1V株基础上经过一系列克隆分离方法而得到的，相对CTN-1V株，CTN-1V-T株在vero等细胞上病变速度更快、滴度略高、效力更高、免疫原性更好，氨基酸序列显示其没有改变CTN-1V株的分子特征，可用于狂犬灭活疫苗的制备。本发明还提供了CTN-1V-T株的全基因组序列。本发明还提供了基于CTN-1V-T株的灭活疫苗的制备过程，结果显示基于CTN-1V-T毒株经过简单工艺就可制备出符合当前药典质量标准的狂犬灭活疫苗。
摘要（英）：

The invention provides a high-titer rabies vaccine CTN-1V-T vero cell adapted strain and also provides a use of the high-titer rabies vaccine CTN-1V-T in preparation of a vero cell purified inactivated rabies vaccine. The CTN-1V-T is obtained by a series of cloning and separation of a CTN-1V strain. Compared with the CTN-1V strain, the CTN-1V-T strain has a faster pathology speed on vero cells, higher titer, higher efficiency and better immunogenicity. An amino acid sequence shows that molecule characteristics of the CTN-1V strain are not changed. The high-titer rabies vaccine CTN-1V-T vero cell adapted strain can be used for preparation of an inactivated rabies vaccine. The invention also provides a whole genome sequence of the CTN-1V-T strain. The invention also provides a preparation method of the CTN-1V-T-based inactivated vaccine. The inactivated rabies vaccine satisfying the current pharmacopeia quality standards can be prepared from the CTN-1V-T strain by simple processes.

0/0