利索-全球专利检索

  1. Login
  2. Sign Up
Invention Grant
CN104833808B 一种miR-486-5p作为生物标记物在胃癌预后预测的方法 失效 - 权利终止 转让

一种miR-486-5p作为生物标记物在胃癌预后预测的方法
Abstract:
本发明公开了一种miR‑486‑5p作为生物标记物在胃癌预后预测的方法，利用miRNA原位杂交方法检测miR‑486‑5p在90例胃癌及癌旁正常组织中的异常表达，评价miR‑486‑5p在胃癌的预后评估作用，在胃癌细胞系中BGC803和SGC7901中，miR‑486‑5P类似物明显抑制胃癌细胞BGC803和SGC7901增殖，促进胃癌细胞BGC803和SGC7901凋亡，miR‑486a‑5p与靶基因FGF9mRNA的3′‑UTR互补，抑制靶基因mRNA的翻译或直接降解靶基因的mRNA，免疫组化检测显示在90例胃癌FGF9的蛋白高表达与miR‑486‑5p低表达密切相关联。
Abstract(English):

The invention discloses a method of miR-486-5p as a biomarker in prognosis prediction of gastric cancer. In the invention, by means of a miRNA on-situ hybridization method for detecting abnormal expression of the miR-486-5p in 90 cases of gastric cancer and adjacent normal tissues, thereby evaluating a prognosis evaluation effect of the miR-486-5p in gastric cancer. In gastric cancer cell lines of BGC803 and SGC7901, a miR-486-5p analogue can significantly inhibit proliferation of the gastric cancer cells BGC803 and SGC7901 and promote apoptosis of the gastric cancer cells BGC803 and SGC7901. The miR-486-5p is complementary with 3'-UTR of a target gene FGF9mRNA so that the miR-486-5p inhibits translation of the target gene mRNA or directly degrades the target gene mRNA. An immunohistochemical detection result proves that in the 90 cases of gastric cancer, low expression of the miR-486-5p is closely relative to protein high expression of gastric cancer FGF9.

Public/Granted literature
Patent Agency Ranking
0/0