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发明公开
CN104838015A 在等位基因特异性PCR中具有经修饰的磷酸酯和碱基的引物 有权

在等位基因特异性PCR中具有经修饰的磷酸酯和碱基的引物
摘要：
本发明包括等位基因特异性扩增的方法，所述方法使用等位基因特异性的寡核苷酸，所述寡核苷酸至少部分地与靶序列的多于一个变体互补，但具有与靶序列的仅一个变体互补的至少一个选择性核苷酸，并且掺有具有在环外氨基处共价修饰的碱基的核苷酸和经修饰的磷酸酯两者。
摘要（英）：

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating both a nucleotide with a base covalently modified at the exocyclic amino group and a modified phosphate.

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