The invention discloses a method for inducing hybrid larch plant regeneration through in vitro culture of adventitious buds, relates to a method for inducing pine plant regeneration through in vitro culture of adventitious buds, and aims at solving the problems that in the existing tissue culture method of hybrid larch, organogenesis is difficult, plant regeneration rate is low, and adventitious bud induction rate is low. The method comprises the following steps: 1 inoculating pretreated zygotic embryos of the hybrid larch into a culture medium, and carrying out dark cultivation or light cultivation until callus tissue is generated; 2 inoculating the callus tissue into the culture medium, carrying out light cultivation until an adventitious buds grow; 3 cutting the callus tissue with the adventitious buds into blocks, transferring into the culture medium, carrying out subculture for once after 3 weeks, carrying out adventitious bud elongation, transferring into the culture medium, and carrying out stooling of the adventitious buds; and 4 transferring the stooled adventitious buds into the culture medium, and carrying out light cultivation to realize rooting of the adventitious buds. According to the method disclosed by the invention, the induction rate of the adventitious buds is 87.73%; the stooling rate is 75.96%; the rooting rate is 45%; and the method is applied for inducing hybrid larch plant regeneration through in vitro culture of adventitious buds.