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发明公开
CN104845984A 苏云金芽胞杆菌表达蛋白对甜菜夜蛾有高毒力的vip3A-1基因 失效 - 权利终止

苏云金芽胞杆菌表达蛋白对甜菜夜蛾有高毒力的vip3A-1基因
摘要：
苏云金芽胞杆菌表达蛋白对甜菜夜蛾有高毒力的vip3A-1基因，属于生物化学技术领域，其表达蛋白对甜菜夜蛾生物活性试验进行初筛和复筛，7d后分别调查死、活虫数，计算校正死亡率和LC50；结果可知，苏云金芽胞杆菌的vip3A-1基因表达蛋白在复筛时对甜菜夜蛾半致死浓度为7.755μg/g(5.683-9.979μg/g)，当表达蛋白浓度达到81μg/g时，所有甜菜夜蛾均死亡，可见苏云金芽胞杆菌的vip3A-1基因表达蛋白对甜菜夜蛾具有高毒力，其特征在于苏云金芽胞杆菌表达蛋白对甜菜夜蛾有高毒力的vip3A-1基因的核苷酸序列如Seq ID No:1所示。
摘要（英）：

The invention discloses a vip3A-1 gene of a bacillus thuringiensis expression protein with high toxicity for asparagus caterpillars, and belongs to the technical field of biochemistry. The expression protein is adopted for primary screening and secondary screening on bioactivity of asparagus caterpillars, the number of dead and live asparagus caterpillars after 7 days, the death rate and LC50 are calculated and corrected, the result shows that the medial lethal concentration of the vip3A-1 gene of the bacillus thuringiensis expression protein to asparagus caterpillars is 7.755mu g/g (5.683-9.979mu g/g) in secondary screening, and when the concentration of the expression protein is up to 81mu g/g, all asparagus caterpillars can be dead, so that the vip3A-1 gene of the bacillus thuringiensis expression protein has high toxicity for asparagus caterpillars, and is characterized in that the nucleotide sequence of the vip3A-1 gene of the bacillus thuringiensis expression protein with high toxicity for asparagus caterpillars is as shown in SEQ ID NO:1.

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