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发明公开
CN104849359A 动物血清中次野鸢尾黄素含量的测定方法 无效 - 驳回

动物血清中次野鸢尾黄素含量的测定方法
摘要：
本发明公开了一种动物血清中次野鸢尾黄素含量的测定方法，将高效液相色谱-质谱联用技术应用于动物血清中次野鸢尾黄素的含量测定，以山奈酚为内标，测定动物血清中次野鸢尾黄素的含量。本发明方法样品处理方法简便易操作，方法回收率满足分析检测要求。采用本发明将HPLC-MS/MS技术应用于动物血清中次野鸢尾黄素的检测，被测组分检测灵敏度提高，分离度好，分析时间短，需样量少，溶剂用量少，含量测定结果准确，重复性、稳定性好。
摘要（英）：

The invention discloses a determination method for the content of irisflorentin in animal serum. The high performance liquid chromatography-mass spectrometry technology is applied to determination of the content of irisflorentin in animal serum; and kaempferol is used as internal standard for determination of the content of irisflorentin in animal serum. According to the method, sample treatment is simple and easy to operate, and a recovery rate meets requirements of analysis and detection; and as the HPLC-MS/MS technology is applied to determination of the content of irisflorentin in animal serum, detection sensitivity of detected components is improved, good resolution is realized, analysis time is short, the desired amount of a sample is small, the usage amount of a solvent is small, content determination results are accurate, and repeatability and good stability are achieved.

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