The invention relates to a Luminex suspension array testing method for the in-vitro immunotoxicity of tobacco smoke. The Luminex suspension array testing method is characterized by comprising the following steps: (1) selecting cells and cell factors; (2) carrying out cell culture; (3) preparing a sample; (4) determining the cell factor; and (5) acquiring and analyzing results. Compared with the prior art, the method has the following characteristics: the flux is high, the cost is low, the speed is high, less samples are required, the expression quantities of 20 different cell factors can be tested simultaneously, the system analysis of cell factor net type adjustment can be realized, and the defects that only one cell factor is tested in one time of testing and the result is interpreted in a one-sidedness manner are overcome; and the cell pretreatment operation is simple and rapid, the testing speed of the cell factor is increased, the testing efficiency is improved, the quantitative testing process of 20 cell factors only requires 4h, less samples are required, only 100mul of cell supernatant is required for testing, and the method is suitable for in-vitro immunotoxicity testing of large-scale samples.