The invention relates to an extraction method for euscaphis konishii active substances, a product and applications for inhibiting increase of cancer cells. The method comprises the main steps: dried and crushed euscaphis konishii materials and ethanol at a material/liquid ratio of 1:10are loaded into an extraction container; an evaporation condensation backflow device is installed, backflow is carried with water bath heating at a temperature of 80 DEG C for 2h every time, for three times, filtration is carried out, the filtrates for the three times are combined, vacuum rotary evaporation collection is carried out, ethanol is recycled, finally drying is carried out at a temperature of 50 DEG C, and a crude extract product S is obtained; the crude extract product S is dissolved in isopyknic distilled water with ultrasonic concussion, extraction is carried out for three times with a ratio of the sample solution to ethyl acetate being 1:3, upper layer ethyl acetate phase liquids are combined, vacuum rotary evaporation collection is carried out, ethyl acetate is recycled, an ethyl acetate phase extract product is obtained, drying is carried out at a temperature of 50 DEG C, an extract product S1 is obtained, the S1 is the provided product. The extraction separation method is simple, resources are abundant, The liver cancer cell inhibition rate of the extract product reaches 81.63%, and is near the liver cancer cell inhibition rate (90.81%) of 5-fluorouracil.