The invention relates to a method for producing virus-free mini-potatoes and a potato atomization cultivation box. The method is characterized by comprising the step a of nutrient solution preparation, the step b of equipment installation and system disinfection and cleaning, the step c of virus-free seedling field planting, the step d of virus-free seedling growth, the step e of stolon inducing, the step f of stolon growth, the step g of tuber inducing, the step h of tuber growth and the step i of mini-potato harvesting. The potato atomization cultivation box is characterized by comprising an upper box body and a nutrient solution recycling pool (8), wherein the upper box body is formed by connecting breathable light-sheltering boxboards (1) which each are in the shape of a Chinese character 'wang', and nutrient solution atomization nozzles (9) are installed at the lower portion of the nutrient solution recycling pool (8).