The invention discloses a portunid water-leaving living freshness preservation method. The method includes the following steps: (1), raising, namely binding big fresh and alive portunid by ropes or rubber bands, placing the fresh and alive portunid in seawater added with keeping-alive agent for temporary raising for 1-2 days, and feeding ozone into the seawater for 0.5-1min every other 5-6h in the period of raising to enable ozone concentration in the seawater to be maintained at 0.1-0.5mg/L; (2), hierarchical cooling anesthesia, namely disposing the portunid after being raised into seawater of 7-8 DEG C for soaking for 1-2min, and placing the portunid in seawater of 1-2 DEG C for soaking for 1-2min; (3), packaging, namely laying a layer of an oxygen producer at the bottom of a packaging box, placing a sponge which fully absorb water on the oxygen producer, arranging a layer of portunid on the sponge, placing a sponge which fully absorb water, arranging a layer of portunid, placing a sponge which fully absorb water, sealing the packaging box, and disposing the packaging box in an environment of 2-5 DEG C for storage. The method is convenient in operation and low in cost, living freshness preservation time of portunid can be increased, survival rate can be increased, and long-distance and long-time alive-keeping transportation of portunid in a no-water packaging form can be realized.