The invention discloses a method for realizing ecological aquaculture method by regulating the water quality of a high-yield aquaculture pond. The method integrates micropore oxygen increasing technology, a fish and vegetable co-existing technology and a water quality regulation micro-ecological agent using technology, so that the water quality of the high-yield aquaculture pond can be improved substantially, the water transparency can be improved, total ammonia nitrogen, nitrite nitrogen, total phosphorus and total nitrogen in water can be reduced, the pH of water can be stabilized, the density of blue algae, green algae and euglena which are harmful to aquaculture can be reduced obviously, diatom, dinoflagellate and cryptomonad which can serve as bait are not influenced obviously, an experimental pond is relatively stable in the aspect of zooplankton, accordingly, micro-ecological balance of the aquaculture water can be improved and stabilized, the yield can be increased, the earnings can be increased for a farmer, and the method can be widely applied to ecological breeding of various types of aquatic animals and plants.