The invention discloses a suppository with a dysmenorrhea treating effect and a preparation method of the suppository. The suppository comprises 3-9 parts of asarum heterotropoide, 6-18 parts of cortex cinnamomi, 10-30 parts of vinegar-fried myrrh, 10-30 parts of olibanum, 15-45 parts of rhizoma corydalis, 10-30 parts of vinegar-fried trogopterus dung, 10-30 parts of scorpions, 10-30 parts of fructus aurantii and 6-18 parts of fried licorice roots. The suppository is scientific and reasonable in raw material proportion and precise and scientific in herbal dosage. According to experimental results, the suppository are excellent in dysmenorrhea treating effect, capable of reaching an effective rate above 90%, quick to take effect, persistent in effect, low in adverse effect and high in safety. By means of filtering the optimal auxiliary materials experimentally and adopting a suppository shaping technology, the prepared suppository are large in drug loading capacity, high in stability, quick in medicine releasing and effect taking, high in persistence and capable of treating dysmenorrhea effectively.