The invention discloses a method for purifying vindesine. The method for purifying the vindesine comprises the following steps of: (1) preparing a chromatographic column from a chromatographic column filler, wherein silica gel, which is swollen by an organic solvent A, serves as the chromatographic column filler; (2) adding a vindesine crude product organic solvent A solution into the chromatographic column; (3) eluting the chromatographic column by a gradient elution method and by sequentially using eluent consisting of organic solvent A/organic solvent B mixed liquid with gradually reduced volume ratio, collecting main component effluent and finally washing out residues; and (4) concentrating under reduced pressure and recycling eluent to obtain high-purity vindesine. The product obtained by the method has high purity. By the method, the purity of the vindesine can reach to more than 90% and the yield of the vindesine can be 40-80%. A stationary phase is stable and can be reused.