The invention discloses a method for symbiotic cultivation of strawberry and agaricus bisporus. The method includes: preparing materials according to an agaricus bisporus cultivation material formula, and obtaining a mushroom cultivation material through early fermentation and late fermentation; applying bran into soil of a strawberry greenhouse in summer, covering the greenhouse with transparent films, utilizing fermentation heat and solar energy to sterilize the soil, ridging, and taking ridge subsoil out for sterilization to prepare mushroom covering soil; cultivating seedlings in a nutrition bowl in summer and autumn to promote flower bud differentiation and new root germination; spreading the material, seeding and earthing in high ridges for mushroom cultivation in autumn, and planting the strawberry seedlings in the nutrition bowl after mycelia grow well in the cultivation material and a covering soil layer; facilitating cultivation management in the strawberry greenhouse, adjusting temperature and humidity, covering the greenhouse, and mulching to prevent or awaken strawberry dormancy, honeybee pollination and induction and development of mushroom fruiting bodies; harvesting at proper time. By the method, high-efficiency and high-quality production of strawberry and mushroom is guaranteed, planting efficiency and benefit are improved, high-value utilization of straw and livestock and poultry manure is realized, and efficient and sustainable development of agriculture is realized.