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发明公开
CN104928181A 一种溶磷多环芳烃降解菌的分离培养基及其制备方法 无效 - 撤回

一种溶磷多环芳烃降解菌的分离培养基及其制备方法
摘要：
本发明公开了一种溶磷多环芳烃降解菌的分离培养基及其制备方法，培养基包括包被物、不溶性含磷化合物、NH4NO3、NaCl、MgSO4、CaCl2、FeCl3和水，包被物、不溶性含磷化合物、NH4NO3、NaCl、MgSO4、CaCl2、FeCl3和水的质量配比的比例为：0.01-0.2:0.1-2:0.5-2:0.5-2:0.1-0.5:0.01-0.05:0.02-0.1:1000。本发明的培养基中含有的磷元素存在形式为包被的不溶性含磷化合物。实验证明，本发明可培养出高浓度的溶磷多环芳烃降解菌，溶磷能力和多环芳烃降解能力明显提高，可用于含油泥沙和土壤等多环芳烃污染环境中溶磷多环芳烃降解菌的高效分离培养。
摘要（英）：

The invention discloses a phosphorus-solubilizing polycyclic aromatic hydrocarbons degrading bacterium isolation medium and a preparation method thereof. The isolation medium comprises a coating object, an insoluble phosphorous compound, NH4NO3, NaC1, MgSO4, CaC12, FeCl3 and water. The mass ratio of the coating object, the insoluble phosphorous compound, NH4NO3, NaC1, MgSO4, CaC12, FeCl3 and water is 0.01-0.2:0.1-2:0.5-2:0.5-2:0.1-0.5:0.01-0.05:0.02-0.1:1000. The existence form of phosphorus contained in the isolation medium is the coated insoluble phosphorous compound. It is indicated by the experiment that phosphorus-solubilizing polycyclic aromatic hydrocarbons degrading bacterium of the high concentration can be cultured, the phosphorus-solubilizing capacity and polycyclic aromatic hydrocarbons degradation capacity are improved remarkably, and the phosphorus-solubilizing polycyclic aromatic hydrocarbons degrading bacterium isolation medium and the preparation method can be used for efficient isolation culturing of phosphorus-solubilizing polycyclic aromatic hydrocarbons degrading bacterium in the polycyclic aromatic hydrocarbons pollution environment such as oily sediment and soil.

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