The invention discloses a clenbuterol hydrochloride molecularly imprinted test paper strip and a preparation method thereof. A nitrocellulose membrane is immerged in a clenbuterol hydrochloride molecularly imprinted polymer solution for soaking; afterwards, the soaked nitrocellulose membrane is oven-dried; the nitrocellulose membrane is washed by using ethyl alcohol to elute template molecules clenbuterol hydrochloride in the imprinted polymer; the washed nitrocellulose membrane is aired; afterwards, the aired nitrocellulose membrane is stuck in a substrate piece with a same hollowed-out area in a pressing manner. By using the test paper strip, the defects that a test paper strip of a same type easily generates a false-negative result and a false-positive result, does not display a quality control line, is too high in detection omission rate, and the like are overcome. A kit prepared on the basis of the test paper strip can be used for carrying out qualitative, semiquantificational and quantificational analysis on the clenbuterol hydrochloride.