The invention discloses a touch screen substrate, a manufacturing method thereof, a touch screen panel and a display device. The touch screen substrate comprises a substrate base plate, first touch control electrodes and second touch control electrodes, wherein the first touch control electrodes and the second touch control electrodes are arranged on the substrate base plate. Orthographic projections, on the substrate base plate, of the first touch control electrodes and the second touch control electrodes are overlapped, and insulating layers are arranged at the region where the orthographic projections, on the substrate base plate, of the first touch control electrodes and the second touch control electrodes are overlapped so as to hinder contact between the first touch control electrodes and the second touch control electrodes. In this way, it can be avoided that when metal etching is performed on the touch control electrodes, metal tips occur, and when metal bridging is performed, due to the excessively-thin overlap joint position of ITO and metal, impedance is prone to being too large, the technology is simple, and touch control is excellent.