The invention relates to the field of medicines for livestock and poultry, and particularly provides a tilmicosin premix and a preparation method thereof. The tilmicosin premix is prepared from the following raw materials in percentage by mass: 5%-50% of tilmicosin, 0.5%-20% of an adhesive and 0.05%-2% of an antioxidant. According to the tilmicosin premix provided by the invention, the medicine odor of the tilmicosin is covered; ingestion of animals is not affected; the tilmicosin premix is wide in raw and supplemental material source, low in cost, and relatively high in absorption speed in an animal body; the bioavailability is greatly improved, so that the dosage of the tilmicosin is reduced; the medicine effect is improved; and the cost is also reduced.