The invention discloses an extraction method of yolk oil. The extraction method comprises the following steps: (1) selecting raw materials, namely, selecting an egg of which the yolk is large and the albumen is less for extraction of yolk oil; (2) breaking the hull of the egg, and stirring the interior of the egg uniformly to obtain a yolk liquid; (3) mixing the yolk liquid with absolute alcohol according to the volume proportion of 1:(3-5), putting the mixture into a water bath kettle, and heating to be 40 to 60 DEG C under the condition of stirring for 30 to 50 minutes' extraction; (4) filtering the solution obtained in the step (3) to obtain a filtrate; (5) heating the filtrate obtained in the step (4) to be 60 to 70 DEG C, and carrying out distilling to recover alcohol, so that a crude yolk oil liquid is obtained; (6) finally, heating the crude yolk oil liquid at 90 to 100 DEG C for 10 to 20 minutes to obtain the yolk oil. The extraction method has the advantages that the yolk oil yield is improved by selecting a high-quality raw material; the extraction method is simple; the cost is low; the extracted yolk oil is high in quality.