The invention relates to a method for detecting isothiazolinone compounds in water-based glue for cigarettes. The method comprises the following steps: (1) adding an extracting agent and an internal standard solution into a sample of the water-based glue for cigarettes, carrying out ultrasonic extraction and centrifuging to obtain liquid supernatant; (2) filtering the liquid supernatant, adding a derivatization reagent to carry out a derivatization reaction, and after the reaction is finished, cooling to room temperature; and (3) carrying out quantitative analysis on the isothiazolinone compounds in a reaction solution of the step (2) by using a GC-MS (Gas Chromatography-Mass Spectrometer). The method provided by the invention can be used for simultaneously detecting the five isothiazolinone compounds in the water-based glue for cigarettes, is rapid and accurate, and can provide technical supports to the quality safety of cigarette products.