The invention discloses an ecological breeding method of breeding penaeus vannamei boone, crucian and chub in a mixed mode. The method includes the steps of preparing a pond, cleaning the pond, inputting water, disinfecting the pond water and richening the water sequentially before throwing in fingerlings and shrimp larvae, throwing in white gold crucian and chub, and then throwing in penaeus vannamei boone larvae. Compared with the prior art, the method has the advantages that the thrown-in white gold crucian does not rob food from the penaeus vannamei boone, the organic content at the bottom of the pond can be greatly decreased, and a substrate is effectively improved; the chub bred in the mixed mode has a good effect on adjusting the balance of algae in a water body, and the water quality can be effectively improved; the substrate and the water quality of the pond can be comprehensively improved by throwing in the fingerlings first, the harm caused by organic matter in bottom mud to the penaeus vannamei boone larvae are further reduced, and the stocking success rate of the penaeus vannamei boone larvae is ensured; the perfect combination of the penaeus vannamei boone, the crucian and the chub has great significance on penaeus vannamei boone breeding environment adjustment, disease prevention and income increase.