The invention relates to the technical field of preparing methods for bio-organic fertilizer, and provides a method for producing organic fertilizer with tobacco waste. The method includes the steps that the tobacco waste is aired, pulverized and exploded at high temperature and pressure, livestock and fowl manure and a microbial agent are added into the tobacco waste, and the mixture is composted and fermented under a natural state to obtain the organic fertilizer. The tobacco waste is treated in a unique integral method, the technology is simple, energy consumption is small, the prepared organic fertilizer has the maximum efficiency, and therefore the method has huge application and popularization value.