The invention provides bacillus sp. ZE01 with collection number of CGMCC NO.10375. The strain is gram-positive bacillus sp. A bacterial colony is milk white and has a dry surface, wrinkles and neat edges. Through VP, gelatinase and glucose oxidation experiments, the strain is positive. Through beta-galactosidase, arginine dihydrolase, lysine decarboxylase, ornithine decarboxylase, tryptophan deiminase and urease experiments, the strain is negative. The strain does not utilize citric acids, does not generate hydrogen sulfide or indole and does not utilize sucrose, arabinose, rhamnose, melibiose, mannitol, inositol, sorbitol or amygdalin. The growth temperature of the strain ranges from 15 DEG C to 45 DEG C, the growth pH of the strain ranges from 5 to 9 and the NaCl concentration for growth of the strain ranges from 0% to 8%. The invention also discloses a method for generating cold temperature-amylase by utilizing the strain ZE01. The cold temperature-amylase has extensive application prospects in the fields of feeds, environmental protection, and the like.