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发明公开
CN105002301A 用于同时检测五种牛病病毒的多重连接探针扩增检测试剂盒及引物和探针 有权

用于同时检测五种牛病病毒的多重连接探针扩增检测试剂盒及引物和探针
摘要：
本发明公开了一种用于检测蓝舌病病毒、牛传染性鼻气管炎病毒、牛病毒性腹泻病毒、牛地方流行性白血病病毒和口蹄疫病毒的多重连接探针扩增检测试剂盒及引物和探针。所述多重连接探针如序列表SEQ ID NO：1至SEQ ID NO：10所示，所述引物如序列表SEQ ID NO：11至SEQ ID NO：12所示。使用本发明所述的引物、探针，和/或包含该引物和探针的多重连接探针扩增检测试剂盒可同时检测蓝舌病病毒、牛传染性鼻气管炎病毒、牛病毒性腹泻病毒、牛地方流行性白血病病毒和口蹄疫病毒5种重要牛病病原，节约了检测时间和成本，有利于疫病的及时确诊。
摘要（英）：

The invention discloses a multiple-connection probe amplification detection kit, primer and probe for simultaneously detecting the bluetongue viruses, the infectious bovine rhinotracheitis viruses, the bovine viral diarrhea viruses, the enzootic bovine leucosis viruses and the foot and mouth disease viruses. The multiple-connection probe is shown in sequence tables from SEQ ID NO:1 to SEQ ID NO:10. The primer is shown in sequence tables from SEQ ID NO:11 to SEQ ID NO:12. The primer, the probe and/or the multiple-connection probe amplification detection kit including the primer and the probe can detect the five vital cow disease pathogenies including the bluetongue viruses, the infectious bovine rhinotracheitis viruses, the bovine viral diarrhea viruses, the enzootic bovine leucosis viruses and the foot and mouth disease viruses at the same time, the detection time and cost are saved, and epidemic diseases can be diagnosed in time.

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