The invention provides a compound in-situ gel preparation with Chinese medicine active part used for pain relieving and a preparation method thereof. The compound in-situ gel preparation comprises the following components by weight percentage: 0.03-1.0% of saffron total glycoside, 0.05-3.0% of paeonol, 0.05-2.0% of total saponins of clematis, 0.05-2.0% of total paeony glycoside, 10-22% of poloxamer 407, 3-18% of poloxamer 188, 17-38% of auxiliary agent and the balance of purified water. According to the invention, the compound in-situ gel preparation with Chinese medicine active part is liquid under normal temperature, and becomes gel due to influence of body temperature and is adhered on the skin, drug efficacy loss due to soup flowing can be effectively avoided, dosage is easily and accurately controlled, concentration of effective medicine can be kept, treatment effect is enhanced, and absorption is faster. The invention also provides a preparation method of the compound in-situ gel preparation with Chinese medicine active part.