The present invention discloses a method for preparing inverse opal photonic crystal fibers. In this method, by means of vertical deposition of colloidal spheres (micron scale or nanoscale), of polystyrene shell-core structured spheres and silica particles, the inverse opal colloidal crystal fiber stripes having a length of about 3.5 cm as well as an adjustable width and thickness is obtained. The invention provides a convenient method and achieves inverse opal photonic crystal fiber stripes with a high yield and a controllable size, and there is no crack on the surface of the fibers or inside the fibers. Furthermore, the inverse opal photonic crystal stripes of the invention can be peeled off from the surface of a glass slide and used conveniently.