The present invention relates to a mouse cover, in particular to a healthcare cooling mouse cover. The mouse cover comprises a cooling jacket, a lead wire port, soft particles, a mouse wheel hole and a liquid injection hole, wherein the upper surface of the cooling jacket is inlaid with the protrusive soft particles; the lead wire port is located at the bottom end of the front side surface of the cooling jacket; the mouse wheel hole is formed in the front end of the cooling jacket and matched in shape with a computer mouse; the liquid injection hole is formed in the back end of the cooling jacket and used for replacing cooling liquid; and the liquid injection hole is sealed with a cover after the cooling liquid is replaced. Compared to an existing technology, the healthcare cooling mouse cover has the advantages that: without affecting normal usage and operation of the mouse, the mouse cover is ensured to be hygienic and bacterial infection and disease transmission are prevented; sweat on a hand will not flow onto the mouse, so that the operation is smoother and the service life of the mouse can be prolonged; when the mouse cover is used, relevant acupoints of a user can be massaged so as to relieve fatigue, and in hot summer, the user can feel cool and comfortable.