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发明公开
CN105039332A HLA基因的组特异性扩增引物、测序分型方法和试剂盒 失效 - 权利终止 转让

HLA基因的组特异性扩增引物、测序分型方法和试剂盒
摘要：
本发明涉及生物医学及免疫遗传学，特别是涉及HLA基因的组特异性扩增引物、测序分型方法和试剂盒，所述PCR扩增引物序列选自：SEQ ID NO.1-28所示的序列，SEQ ID NO.29-64所示的序列和SEQ ID NO.65-94所示的序列。本申请通过对HLA基因进行血清分类设计组特异性扩增引物，可对HLA-A、-B和-C所有等位基因进行测序分型；对HLA-A、-B和-C全长序列进行扩增测序，可有效解决模棱两可，达到绝对高分，数据更加准确可靠。
摘要（英）：

The invention relates to biomedicines and immunological genetics and particularly relates to a group-specific amplification primer, sequence-based typing method and a kit for HLA genes. Sequences of a PCR amplification primer are selected from sequences represented by SEQ ID NO.1-28, sequences represented by SEQ ID NO.29-64 and sequences represented by SEQ ID NO.65-94. By designing the group-specific amplification primer by carrying out serum classification on the HLA genes, all allelic genes including HLA-A, -B and -C can be subjected to sequence-based typing; by carrying out amplification sequence on full-length sequences of genes HLA-A, -B and -C, the ambiguity can be effectively solved, an absolute high score is achieved, and data is relatively accurate and reliable.

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