The invention relates to a codon optimized phosphocholine cytidylyltransferase cct gene and expression thereof, wherein the cct gene is constructed on the basis of escherichia coli codon preference. The optimized cct gene is obtained through a total synthesis method, the nucleotide sequence of the gene is represented by SEQ ID No.1, and the sequence of coding amino acid is represented by SEQ ID No.2. The length of the gene is 1275 bp, and encodes 424 amino acids. The homology between optimized cct gene and wild cct gene is 76.6%; the optimized cct gene is connected to a carrier pET-29a(+) to obtain a recombinant carrier pET-29a-cct-op, and the recombinant carrier is converted to escherichia coli. After IPTG inducible expression and enzyme activity detection, an engineering bacterium strain, which has been modified by preference and can efficiently express phosphocholine cytidylyltransferase, is obtained. The invention has the advantages that brewer's yeast cct gene is redesigned according to the preference of escherichia coli codon so as to improve the efficiency of escherichia coli on expressing phosphocholine cytidylyltransferase, the gene can be used to produce phosphocholine, the industrial output can be increased, and the economic profits for enterprises are increased.