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发明公开
CN105039585A 用于鸡传染性支气管炎病毒RT-PCR检测的引物及含有该引物的试剂盒及其检测方法 无效 - 驳回

用于鸡传染性支气管炎病毒RT-PCR检测的引物及含有该引物的试剂盒及其检测方法
摘要：
本发明涉及一种用于鸡传染性支气管炎病毒荧光定量RT-PCR检测的引物及含有该引物的检测试剂盒及其检测方法。包括用于扩增鸡传染性支气管炎病毒（IBV）5′端非编码区保守序列的特异性引物对，其中所述的引物对由引物1和引物2组成，所述引物1为序列表中序列1或序列2或序列3所示的单链DNA，所述引物2为序列表中序列4或序列5或序列6所示的单链DNA。通过特异性、灵敏度、稳定性和重复性实验，证明该试剂盒只能特异性扩增IBV核酸，在1×102～1×107个拷贝·μL-1范围内具有良好的线性关系，灵敏度达到102个拷贝·μL-1。本发明适用于各种组织、细胞、体液样品及疫苗生产中间产品中IBV核酸的定量检测。
摘要（英）：

The invention relates to a primer used in RT-PCR detection of chicken infectious bronchitis virus, a kit comprising the primer and a detection method thereof. The primer includes a specific primer pair used for amplifying a 5-terminal non-coding region conserved sequence of chicken infectious bronchitis virus (IBV). The specific primer pair is composed of a primer 1 and a primer 2, wherein the primer 1 is a single-chain DNA represented in the Seq ID No.1, the Seq ID No.2 or the Seq ID No.3 in the sequence table, and the primer 2 is a single-chain DNA represented in the Seq ID No.4, the Seq ID No.5 or the Seq ID No.6 in the sequence table. Tests comprising specificity, sensitivity, stability and repeatability prove that the kit can only specifically amplify IBV nucleic acid and is excellent in linearity relationship within the range of 1*10<2>-1*10<7> copies/[mu]L. The sensitivity of the primer reaches 10<2> copies/[mu]L. The primer is suitable for quantitative detection of various tissue, cell, body fluid samples and vaccine production intermediate products.

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