The invention belongs to the technology of comprehensive recycling of complex secondary nonferrous metal resources, and particularly relates to an enrichment and separation method of cobalt in waste circuit board multi-metal hybrid resources. The method includes the steps that firstly, a multi-metal complex mixture containing cobalt is obtained from a waste circuit board through smashing and sorting; a collecting agent is added into the multi-metal complex mixture; the prepared multi-metal complex mixture is placed in a graphite crucible of a vacuum furnace; after the metal mixture is completely melted, trace enrichment reagent niobium or boron is added to regulate the separation rate of liquid copper and liquid iron; a liquid copper and liquid iron mixed melt is a separation melt, the upper layer of the separation melt is the liquid iron, and the lower layer of the separation melt is the liquid copper; and the liquid iron gathering cobalt on the upper layer is poured out, and cobalt is separated from the multi-metal complex mixture of the waste circuit board to be recycled. The method is simple and easy to implement and has the characteristics of being low in cost, comprehensive, efficient, free of pollution and the like.