The invention provides a wireless throughput testing method. The method comprises the following steps of: newly configuring N sub-networks with different service set identifiers for a throughput testing device, wherein each service set identifier is corresponding to a different virtual local area network with an exclusive network segment; enabling a first terminal to connect to the N sub-networks with different service set identifiers, obtaining corresponding network attribute of the first terminal according to the exclusive network segment; newly configuring a transmitting-receiving port of the throughput testing device, and connecting the corresponding virtual local area network; newly configuring a receiving port and M forwarding ports of an interactive device, and connecting the corresponding local area network; enabling a second terminal to connect to the corresponding forwarding port, configuring the network attribute according to the exclusive network segment of the second terminal; determining a testing terminal from N first terminals, configuring wireless throughput testing script for the testing terminal, and running the wireless throughput testing script within a preset period of time to obtain the wireless throughput. With the method and the system, the wireless throughput can be tested and obtained accurately by statistics, and batch test of throughput is realized.