The present invention provides a physical isolation transmission method based on a two-dimension code. The physical isolation transmission method based on the two-dimension code comprises the steps of using a first network terminal to carry out the automatic block processing on the original data needing to be sent, and obtaining the block data; separately encrypting the block data and converting the data into the corresponding two-dimension code pictures; displaying the two-dimension code pictures orderly according to the block serial numbers on the first network terminal to obtain the combined two-dimension code pictures; using a second network terminal to scan the combined two-dimension code pictures; decrypting the acquired combined two-dimension code pictures and separating into the corresponding two-dimension code pictures; analyzing the separated two-dimension code pictures to obtain the corresponding block data and write the data in a cache file; using the second network terminal to merge the all received block data to obtain the completely merged original data and store. The advantages of the present invention are that: the physical isolation transmission method based on the two-dimension code enables the data transmission reliability and accuracy to be improved, solves the problem that the data synchronization is difficult due to the isolation of the internal and external networks, and guarantees the security and efficiency of the physical isolation transmission.