The invention discloses an oliopeptide with anti-lung cancer effect. The amino acid sequence of the oliopeptide is shown as SEQ ID NO.1. Experiments show that the oliopeptide disclosed by the invention has selective killing ability to lung cancer cells, and has an obvious anti-bacterial effect on Gram-negative bacteria. The anti-bacterial effect on fungi is similar to that on the Gram-negative bacteria. The oliopeptide is expected to be directly used for preparing an anti-lung cancer anti-bacterial medicine and solving the problem of drug resistance; the application prospect is considerable.